Journal: The Journal of Neuroscience

Article Title: Impact of the NGF Maturation and Degradation Pathway on the Cortical Cholinergic System Phenotype

doi: 10.1523/JNEUROSCI.1144-11.2012

Figure Lengend Snippet: Antibodies used for the labeling of cortical synaptic boutons

Article Snippet: The sections were washed 3× 10 min in PBS, mounted on gelatin subbed slides, dried overnight at 4°C, and coverslipped with Aqua Polymount (Polysciences). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Anatomical marker Primary antibody Serum used for blocking step and antibody dilutions Secondary antibody VAChT (expressed in cholinergic varicosities) Goat anti-VAChT; 1:10,000 (Millipore), polyclonal Normal rabbit serum (Jackson ImmunoResearch) Biotinylated rabbit anti-goat; 1:200 (Vector Labs) TH (expressed in both dopaminergic and noradrenergic varicosities) Rabbit anti-TH; 1:3000 (Millipore), polyclonal Normal goat serum (Jackson ImmunoResearch) Alexa Fluor 488-conjugated goat anti-rabbit; 1:800 (Invitrogen) DBH (expressed in noradrenergic varicosities) Mouse anti-DBH; 1:25 (MediMabs), monoclonal Normal donkey serum (Jackson ImmunoResearch) Rhodamine Red X-conjugated donkey anti-mouse; 1:200 (Jackson ImmunoResearch) VGluT1 (expressed in glutamatergic varicosities) Mouse anti-VGluT1; 1:100 (characterized in Bell et al. (2006) ; MediMabs), monoclonal Normal donkey serum (Jackson ImmunoResearch) Alexa Fluro 488-conjugated donkey anti-mouse; 1:800 (Invitrogen) GAD 65 (expressed in GABAergic varicosities) Mouse anti-GAD-65; 1:1000 (Millipore), monoclonal Normal donkey serum (Jackson ImmunoResearch) Alexa Fluor 488-conjugated donkey anti-mouse; 1:800 (Invitrogen) Neurotrophic TrkA high-affinity mNGF receptor (expressed on cholinergic axon) Polyclonal goat anti-TrkA; 1:100 (R&D Systems) Normal donkey serum (Jackson ImmunoResearch) Rhodamine Red X-conjugated donkey anti-goat; 1:200 (Jackson ImmunoResearch) Open in a separate window Antibodies used for the labeling of cortical synaptic boutons

Techniques: Labeling, Marker, Blocking Assay, Plasmid Preparation

Journal:

Article Title: Entry of Amphotropic and 10A1 Pseudotyped Murine Retroviruses Is Restricted in Hematopoietic Stem Cell Lines

doi:

Figure Lengend Snippet: Binding of amphotropic and 10A1 pseudotyped MLV to fibroblasts and hematopoietic cell lines. Cells were incubated with virus supernatants. Total virions, as well as the viral glycoprotein gp70SU, bound to the cell surface were then stained with an anti-gp70 antibody, followed by binding of a biotinylated secondary antibody and streptavidin-phycoerythrin. The cells were then analyzed on a flow cytometer.

Article Snippet: For the 10A1 strain, a goat antiserum to MLV gp70 ( 35 ) followed by a biotinylated donkey anti-goat antibody (Laboserv, Giessen, Germany) was used.

Techniques: Binding Assay, Incubation, Staining, Flow Cytometry